Control predictivo de modelos borrosos Takagi-Sugeno mediante funciones de Lyapunov contractivas

Desarrollar una técnica de control predictivo (MPC) aplicado a modelos borrosos Takagi-Sugeno (TS), haciendo uso de los conceptos de estabilidad de Lyapunov y las funciones contractivas

Polytopic invariant and contractive sets for closed-loop discrete fuzzy systems

In this work a procedure for obtaining polytopic lambda-contractive sets for Takagi Sugeno fuzzy systems is presented, adapting well-known algorithms from literature on discrete-time linear difference inclusions (LDI) to multi-dimensional summations. As a complexity parameter increases, these sets tend to the maximal invariant set of the system when no information on the shape of the membership functions is available. lambda-contractive sets are naturally associated to level sets of polyhedral Lyapunov functions proving a decay-rate of lambda. The paper proves that the proposed algorithm obtains better results than a class of Lyapunov methods for the same complexity degree: if such a Lyapunov function exists, the proposed algorithm converges in a finite number of steps and proves a larger lambda-contractive set.This work has been supported by Projects DPI2011-27845-C02-01 and DPI2011-27845-C02-02, both from Spanish Government.Arino, C.; Perez, E.; Sala Piqueras, A.; Bedate, F. (2014). Polytopic invariant and contractive sets for closed-loop discrete fuzzy systems. Journal of The Franklin Institute. 351(7):3559-3576. https://doi.org/10.1016/j.jfranklin.2014.03.014S35593576351

Methylation status of the p15, p16 and MGMT promoter genes in primary cutaneous T-cell lymphomas

p15(INK4b), p16(INK4a) and O(6)-methylguanine DNA methyltransferase (MGMT) gene hypermethylation was studied in 22 patients with primary cutaneous T-cell lymphomas (CTCL). p15(INK4b) and p16(INK4a) inactivation is present in early and advanced disease and seems to be independent of disease stage. MGMT inactivation may play a pathogenetic role in a subset of CTCL

Absence of MALT1 traslocation in primary cutaneous marginal zone B-cell lymphoma

The implication of MALT1 gene in the pathogenesis of primary cutaneous marginal zone B-cell lymphomas (PCMZL) has been a matter of controversy. We examined the presence of MALT1 translocations in a series of 23 PCMZL. FISH assay with a MALT1 dual color break apart translocation probe revealed the absence of MALT1 translocations in all cases

Comparative Analysis of TCR-gamma Gene Rearrangements by Genescan and Polyacrylamide Gel-electrophoresis in Cutaneous T-cell Lymphoma

Demonstrating T-cell clonality has become an important approach supporting a diagnosis of malignant T-cell neoplasms. A comparative study between Genescan analysis, polyacrylamide gel and agarose gel electrophoresis in visualizing X-cell receptor gamma gene rearrangement was performed on 25 biopsy specimens from 18 patients with different forms of cutaneous T-cell lymphomas. Clonality was detected in 17 biopsy specimens when PCR products were evaluated by Genescan analysis. Seventeen showed discrete bands when visualized in polyacrylamide gel and 14 cases were clonal when visualized with agarose gel. In five cases, a clonal population was seen in the gels, but not with Genescan. On sequencing the PCR products we demonstrated nonclonality of these five samples. Our results confirm that PCR-Genescan is a useful, reliable and specific screening method for detecting dominant clones in patients with T-cell lymphoma

Lymphomatoid papulosis associated with mycosis fungoides. A clinicopathological and molecular study of 12 cases

The association of mycosis fungoides and a primary cutaneous CD30+ lymphoproliferative disorder has been reported and probably represents different clinical aspects of a unique T-cell monoclonal expansion. In this study, 12 patients (6 men and 6 women) presented with lymphomatoid papulosis and mycosis fungoides. A TCRgamma gene rearrangement study was performed by an automated high-resolution PCR fragment analysis method on skin biopsy specimens taken from the different clinical lesions in each patient. An indolent clinical course was observed in the majority of patients. T-cell clonality was identified in 7 of 12 lymphomatoid papulosis lesions (58%) and in 6 skin biopsies of plaque stage mycosis fungoides (50%). In each individual case, where T-cell clonality was detected, both mycosis fungoides and lymphomatoid papulosis specimens exhibited an identical peak pattern by automated high-resolution PCR fragment analysis, confirming a common clonal origin. Only one case showed a clonal TCRgamma rearrangement from the lymphomatoid papulosis lesion, which could not be demonstrated in the mycosis fungoides specimen. The demonstration of an identical clone seems to confirm that both disorders are different clinical manifestations of a unique T-cell monoclonal proliferation. Our results also seem to confirm that the association of mycosis fungoides with a primary cutaneous CD30+ lymphoproliferative disorder usually carries a favourable prognosis

MicroRNA expression profiling and DNA methylation signature for deregulated microRNA in cutaneous T-cell lymphoma

MicroRNAs usually regulate gene expression negatively, and aberrant expression has been involved in the development of several types of cancers. Microarray profiling of microRNA expression was performed to define a microRNA signature in a series of mycosis fungoides tumor stage (MFt, n=21) and CD30+ primary cutaneous anaplastic large cell lymphoma (CD30+ cALCL, n=11) samples in comparison with inflammatory dermatoses (ID, n=5). Supervised clustering confirmed a distinctive microRNA profile for cutaneous T-cell lymphoma (CTCL) with respect to ID. A 40 microRNA signature was found in MFt including upregulated onco-microRNAs (miR-146a, miR-142-3p/5p, miR-21, miR-181a/b, and miR-155) and downregulated tumor-suppressor microRNAs (miR-200ab/429 cluster, miR-10b, miR-193b, miR-141/200c, and miR-23b/27b). Regarding CD30+ cALCL, 39 differentially expressed microRNAs were identified. Particularly, overexpression of miR-155, miR-21, or miR-142-3p/5p and downregulation of the miR-141/200c clusters were observed. DNA methylation in microRNA gene promoters, as expression regulatory mechanism for deregulated microRNAs, was analyzed using Infinium 450K array and approximately one-third of the differentially expressed microRNAs showed significant DNA methylation differences. Two different microRNA methylation signatures for MFt and CD30+ cALCL were found. Correlation analysis showed an inverse relationship for microRNA promoter methylation and microRNA expression. These results reveal a subgroup-specific epigenetically regulated microRNA signatures for MFt and CD30+ cALCL patients

Association of APOA5 and APOC3 genetic polymorphisms with severity of hypertriglyceridemia in patients with cutaneous T-Cell lymphoma treated with bexarotene

Importance: hypertriglyceridemia is the most frequent and limiting adverse effect of bexarotene therapy in cutaneous T-cell lymphoma (CTCL). Despite standard prophylactic measures, there is a wide variability in the severity of this complication, which could be associated with both genetic and environmental factors. Objectives: to analyze the association between genetic polymorphisms of apolipoprotein genes APOA5, APOC3, and APOE and the severity of hypertriglyceridemia during bexarotene therapy and to optimize patient selection for bexarotene therapy based on adverse effect profile. Design, Setting, and Participants: this case series study was conducted in 12 university referral hospitals in Spain from September 17, 2014, to February 6, 2015. One hundred twenty-five patients with a confirmed diagnosis of CTCL who had received bexarotene therapy for at least 3 months were enrolled. Nine patients were excluded owing to missing analytic triglyceride level data, leaving a study group of 116 patients. Data on demographic and cardiovascular risk factor were collected, and a complete blood analysis, including lipid profile and genetic analysis from a saliva sample, was performed. Main Outcomes and Measures: primary outcomes were the maximal triglyceride levels reported in association with the minor alleles of the polymorphisms studied. Results: among 116 patients, the mean (SD) age was 61.2 (14.7) years, 69 (59.5%) were men, and 85 (73.2%) had mycosis fungoides, the most prevalent form of CTCL. During bexarotene therapy, 96 patients (82.7%) experienced hypertriglyceridemia, which was severe or extreme in 8 of these patients (8.3%). Patients who carried minor alleles of the polymorphisms did not show significant differences in baseline triglyceride concentrations. After bexarotene treatment, carriers of at least 1 of the 2 minor alleles of APOA5 c.-1131T>C and APOC3 c.*40C>G showed lower levels of triglycerides than noncarriers (mean [SD], 241.59 [169.91] vs 330.97 [169.03] mg/dL, respectively; P = .02). Conclusions and Relevance: these results indicate that the screening of APOA5 and APOC3 genotypes may be useful to estimate changes in triglyceride concentrations during bexarotene treatment in patients with CTCL and also to identify the best candidates for bexarotene therapy based on the expected adverse effect profile

Asymptotically exact stabilisation for constrained discrete Takagi-Sugeno systems via set-invariance

[EN] Given a Takagi-Sugeno (TS) system, this paper proposes a novel methodology to obtain the state feedback controller guaranteeing, asymptotically as a Polya-related complexity parameter grows, the largest (membership-shape independent) possible domain-of-attraction with contraction-rate performance lambda, based on polyhedral lambda-contractive sets from constrained linear systems literature. The resulting controller is valid for any realisation of the memberships, as usual in most TS literature. For a finite complexity parameter, an inner estimate of such largest set is obtained; the frontier of such approximation can be understood as the level set of a polyhedral control-Lyapunov function. Convergence of a proposed iterative algorithm is asymptotically necessary and sufficient for TS system stabilisation: for a high-enough value of the complexity parameter, any conceivable shape-independent Lyapunov controller design procedure will yield a proven domain of attraction smaller or equal to the algorithm's output. (C) 2016 Elsevier B.V. All rights reserved.This work has been supported by grants DPI2015-70433- P and DPI2016-81002-R, from Spanish Government (MINECO) and grant PROMETEOII/2013/004 from Generalitat Valenciana.Ariño-Latorre, CV.; Sala, A.; Pérez Soler, E.; Bedate Boluda, F.; Querol-Ferrer, A. (2017). Asymptotically exact stabilisation for constrained discrete Takagi-Sugeno systems via set-invariance. Fuzzy Sets and Systems. 316:117-138. https://doi.org/10.1016/j.fss.2016.10.004S11713831

Efficacy of MSC for steroid-refractory acute GVHD associates with MSC donor age and a defined molecular profile

Contains fulltext : 229413.pdf (Publisher’s version ) (Closed access